Quality Control
Functionality tested in Real-time PCR for specificity, sensitivity and reproducibility for each primer sets using hematopoietic cell lines.
BCR-ABL Service
NanoCinna Pharmacogenomics Center
BCR-ABL or Philadelphia (Ph) chromosome
Philadelphia chromosome or Philadelphia translocation is a specific chromosomal abnormality that is associated with chronic myelogenous leukemia (CML). It is the result of a reciprocal translocation between chromosome 9 and 22, and is specifically designated t(9;22)(q34;q11). The presence of this translocation is a highly sensitive test for CML, since 95% of people with CML have this abnormality. The result of the translocation is the oncogenic BCR-ABL gene fusion, located on the shorter derivative 22 chromosome. This gene encodes the Bcr-abl fusion protein. Depending on the precise location of the fusion the molecular weight of the protein can range from 190 to 230 kDa. For this reason bcr-abl is sometimes called p190, p210 or p230. Identification of the transcript (p190, p210 or p230) does not reveal the type of junction but this information is very important for classification of patients in clinical trials. Most identification kits do not explore p230 transcripts and are unable to determine exotic breakpoints. We have thus developed a clinical molecular diagnosis assay based on RT-PCR, able to identify all types of the BCR-ABL transcripts and characterize all of the possible transcript junctions in a rapid and precise manner.
Application
Identification of p190 transcripts
Identification of 3 types p210 transcripts
Identification of p230 transcripts
Identification of p203 transcripts
Identification of type of junction(s) at the same time
Sampling Condition
Bone marrow or blood sample needed,
for more information please contact us.
Catalog Number
#S1370 BCR-ABL qualitative assay
#S1374 BCR-ABL quantitative assay
#S1370 and #S1374 description
Diagnosis of CML based on detection of the Philadelphia (Ph) chromosome or the BCR-ABL gene by 3 sense primers and 1 reverse primer
Characterization of all types (6 types) of BCR-ABL transcripts with Real-time PCR in two qualitative and quantitative steps.
The quantitative method determines ratio of BCR-ABL Vs ABL or GusB genes.
Global frequency in CML patients
frequency in Iranian CML patients
